Do You Know How To Choose A Membrane Filter?

In the laboratory, no matter it is an inorganic experiment, organic experiment, or physical and chemical experiment, overkill operation is essential. How to choose the syringe filter membrane and filter reasonably and quickly is a skill that every experimental worker must master. Laboratory filtration selection guide is what we should know.

Recognize the importance of filtering. Filtering protects chromatographic systems and columns, prolongs their service life, and improves the accuracy of data. Filtration eliminates pressure fluctuations due to friction particles and baseline fluctuations due to irregular impurities. The interference of bubbles to the detection system can be eliminated.

Therefore, a membrane filter is usually used to filter the mobile phase and a syringe filter is used to filter the sample. Membrane manufacturer provide related parameters and common sense, let us know the first.

PP membrane fliter hydrophobic ptfe membrane filter hydrophilic ptfe membrane filter

Related parameters and common sense of membrane filter

In order to better understand syringe filter membrane selection, now we first learn the relevant parameters and common sense of the lower membrane filter.

1. Air flux
Is a method of measuring air passing through a filter. That is, in the case of different pressure, different porosity, and different device area, the flow of air through.

2. The bubble point
In the microporous membrane industry, the use of a specific liquid to wet the roll membrane, and at a specific temperature, the need to squeeze out of the membrane hole liquid minimum pressure.

3. Absolute aperture
The absolute pore size class is defined by identifying pore size by 100% interception of challenging bacteria of a particular size under very stringent test conditions. Among the conditions that must be specified are the measurement of organic (or molecular) size and concentration, measurement of pressure, and inspection.

4. Filter function
Filters are defined in terms of their overdose and blocking particle size at specific pressures. In general, the lower the obstruction and pressure, the more effective the filter.

5. Hydrophobic and hydrophilic membrane
Hydrophilic membrane filter usually has a special chemical layer that allows the membrane to be infiltrated by water; Hydrophobicity is a reference for repulsion to water. Hydrophobic membrane filter rarely does not absorb water at all. In observation, small droplets of water can be seen to stay on the surface of the filter membrane without being absorbed by the surface and diffused into the water surface. The size of hydrophobicity depends on the aperture of the filter material and the characteristics of the membrane filter material.

6. Filter material life
The maximum lifetime of the filter under certain operating conditions. It depends on many factors, such as the nature of the filtrate, the operating temperature, and the choice of filter material.

To know more about Membrane Material And Performance Characteristics Of Filter Membrane

nylon membrane filter pvdf membrane filter how to choose fliter membrane

7. Flow rate and pore rate
Flow rate is the amount of filtrate passing through the membrane per unit of time at a specific temperature and pressure. The flow rate is closely related to the surface properties of the membrane filter. Flow rate and pore rates are two important parameters of filter material and design performance. This performance depends on the following aspects :

(1) Pressure difference is the pressure difference between inlet and outlet infiltration. When the filter is fully loaded, the filtration pressure difference increases.
(2) Viscosity: viscosity determines the flow of the liquid. The higher the viscosity of the liquid (at a given temperature and pressure), the lower the flow rate. The higher the pressure required to achieve the same flow rate.
(3) Pore rate: refers to the proportion of the volume of all holes in the filter membrane to the total volume of the filter membrane. Usually, the filter membrane has a 50-90% pore area, and the flow rate is directly related to the pore rate of the membrane.

How to select the right membrane syringe filter? You should be considered in the selection of the filter membrane, the following is filter membrane selection guide:

Syringe filter membrane selection

1. Filter material of the filter membrane

When choosing a filter membrane, chemical compatibility should be considered first. Is the filter resistant to acid, alkali, organic solvents, etc?

• PTFE membrane is compatible with almost all solvents, acids, and alkalis.
• PP membrane have good compatibility, stable chemical performance, acid and alkali resistance
• Nylon 66 membrane is compatible with most organic solvents and water-based solvents; but when used with strong acids,
Dichloromethane and DMF are not recommended.
• Cellulose acetate membrane is not compatible with organic solvents. They are very suitable for aqueous solutions and are especially recommended for protein and protein-related samples.

2. The pore size of the filter membrane

For chromatographic column systems using fillers of 3um or greater size, membrane filter 0.45 or filter membrane can be used. For chromatographic systems with fillers less than 3um or involving microbial growth, a 0.2um filter is recommended. For the difficult turbidity solution, a 1-5um filter membrane can be used for pre-filtration, and then the corresponding filter membrane can be used for further filtration.

3. Characteristics of samples

(1) Highly corrosive organic solvents: generally using a hydrophobic membrane.
(2) Water-borne sample: water-wet diaphragm is selected. It has an affinity for water and is suitable for filtering water-based solutions. Available filter membranes are mixed cellulose membrane, polyethersulfone (PESM), NylonM, etc.
(3) Protein solution: choose the filter membrane with low protein adsorption, such as the PVDF filter membrane.
(4) Ion chromatography: PES membrane is generally considered to be suitable for filtration of solutions with low inorganic ions.

4. When selecting a syringe filter

In addition to the above factors, consider the sample size (that is, the size of the syringe filter to choose): usually, if the sample size is less than 2ml, a miniature filter head with a diameter of 4mm should be selected. The sample size is between 2 and 10ml, and a filter head with a diameter of 13mm is selected. When the sample size is greater than 10ml, a filter head with a diameter of 25mm is selected.

Click here to know The Differences Between PVDF and PTFE Membrane
Click here to know Working Principle of Membrane Filter.
Click here to know 10 Steps To Clean Membrane Filter.