Filter membranes are divided into hydrophilic and hydrophobic membranes. Filter membrane has accurate pore size distribution, high strength, and flexibility, and the product can ensure repeatability and consistency. The pores of the membrane filter sterilization act as small capillaries. When the hydrophilic membrane comes into contact with water, capillary action related to surface tension causes water to spontaneously enter and fill the pores. In this way, the filter membrane is easily wetted and allows a large amount of water to flow through the holes.
Once wetted, the hydrophilic membrane will not allow air or other gases to flow in large amounts unless they are applied at a pressure greater than the bubble point of the membrane. When the hydrophobic membrane comes into contact with water, the surface tension will act to drive the water out of the pores. Unless the applied water pressure is greater than the water inlet pressure of the hydrophobic membrane, water will not enter the pores and the hydrophobic membrane will become a barrier to water flow.
If you want to cell culture, then CA, PES membrane filter sterilization is your choice. If your experiment requires solvent filtration, gas filtration, or venting, then a PTFE disk membrane filter is just what you need. 0.45 disc nylon membrane filter 47mm is ideal for filtering Ameno acid solutions. GF membrane filter is suitable for pre-filtration and serial filtration. If you are not sure what membrane filter to choose, the membrane filter factory technical support team, equipped with a full analytical testing lab, can help you through the process. As a membrane filter factory, we provide a wide range of filter membranes with a wide range of pore sizes, aseptic and autoclaved packaging membranes, and OEM services.
In a specific experiment, you should choose a filter membrane and filter with a suitable pore size according to your experimental needs for filtration purposes.
0.1μm: Hydrophilic and hydrophobic membranes can remove mycoplasma from the sample. 0.22μm: The filter membrane can remove 99.99% of bacteria and microorganisms, meeting the requirements of GMP or Pharmacopoeia for sterilization, and can also remove very fine particles in samples and mobile phases. 0.45μm: The filter membrane can filter out most of the bacteria and microorganisms, and can meet the general chromatographic requirements for the filtration of conventional samples and the same flow. 0.8μm and above: The filter membrane can filter larger particles of impurities or the pretreatment of difficult or turbid samples. After the pretreatment, the corresponding filter membrane can be selected for filtration. 1-5μm: To filter impurities with larger particles, or for pretreatment of difficult-to-handle turbid solutions, it can be filtered with a 1-5um filter first and then filtered with the corresponding filter.